Prediction of protein-RNA binding sites by a random forest method with combined features

doi:10.1093/bioinformatics/btq253

CSpace > 应用数学研究所

	Prediction of protein-RNA binding sites by a random forest method with combined features
	Liu, Zhi-Ping 1; Wu, Ling-Yun2 ; Wang, Yong2 ; Zhang, Xiang-Sun2 ; Chen, Luonan 1
	2010-07-01
发表期刊	BIOINFORMATICS
ISSN	1367-4803
卷号	26 期号:13 页码:1616-1622
摘要	Motivation: Protein-RNA interactions play a key role in a number of biological processes, such as protein synthesis, mRNA processing, mRNA assembly, ribosome function and eukaryotic spliceosomes. As a result, a reliable identification of RNA binding site of a protein is important for functional annotation and site-directed mutagenesis. Accumulated data of experimental protein-RNA interactions reveal that a RNA binding residue with different neighbor amino acids often exhibits different preferences for its RNA partners, which in turn can be assessed by the interacting interdependence of the amino acid fragment and RNA nucleotide. Results: In this work, we propose a novel classification method to identify the RNA binding sites in proteins by combining a new interacting feature (interaction propensity) with other sequence- and structure-based features. Specifically, the interaction propensity represents a binding specificity of a protein residue to the interacting RNA nucleotide by considering its two-side neighborhood in a protein residue triplet. The sequence as well as the structure-based features of the residues are combined together to discriminate the interaction propensity of amino acids with RNA. We predict RNA interacting residues in proteins by implementing a well-built random forest classifier. The experiments show that our method is able to detect the annotated protein-RNA interaction sites in a high accuracy. Our method achieves an accuracy of 84.5%, F-measure of 0.85 and AUC of 0.92 prediction of the RNA binding residues for a dataset containing 205 non-homologous RNA binding proteins, and also outperforms several existing RNA binding residue predictors, such as RNABindR, BindN, RNAProB and PPRint, and some alternative machine learning methods, such as support vector machine, naive Bayes and neural network in the comparison study. Furthermore, we provide some biological insights into the roles of sequences and structures in protein-RNA interactions by both evaluating the importance of features for their contributions in predictive accuracy and analyzing the binding patterns of interacting residues.
DOI	10.1093/bioinformatics/btq253
语种	英语
资助项目	Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences[2009CSP002] ; National Natural Science Foundation of China[10631070] ; National Natural Science Foundation of China[60873205] ; Ministry of Science and Technology of China[2006CB503905]
WOS研究方向	Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology ; Computer Science ; Mathematical & Computational Biology ; Mathematics
WOS类目	Biochemical Research Methods ; Biotechnology & Applied Microbiology ; Computer Science, Interdisciplinary Applications ; Mathematical & Computational Biology ; Statistics & Probability
WOS记录号	WOS:000278967500006
出版者	OXFORD UNIV PRESS
引用统计
文献类型	期刊论文
条目标识符	http://ir.amss.ac.cn/handle/2S8OKBNM/9548
专题	应用数学研究所
通讯作者	Chen, Luonan
作者单位	1.Chinese Acad Sci, Shanghai Inst Biol Sci, SIBS Novo Nordisk Translat Res Ctr PreDiabet, Key Lab Syst Biol, Shanghai 200031, Peoples R China 2.Chinese Acad Sci, Acad Math & Syst Sci, Beijing 100190, Peoples R China
推荐引用方式 GB/T 7714	Liu, Zhi-Ping,Wu, Ling-Yun,Wang, Yong,et al. Prediction of protein-RNA binding sites by a random forest method with combined features[J]. BIOINFORMATICS,2010,26(13):1616-1622.
APA	Liu, Zhi-Ping,Wu, Ling-Yun,Wang, Yong,Zhang, Xiang-Sun,&Chen, Luonan.(2010).Prediction of protein-RNA binding sites by a random forest method with combined features.BIOINFORMATICS,26(13),1616-1622.
MLA	Liu, Zhi-Ping,et al."Prediction of protein-RNA binding sites by a random forest method with combined features".BIOINFORMATICS 26.13(2010):1616-1622.