Conic shapes have higher sensitivity than cylindrical ones in nanopore DNA sequencing

doi:10.1038/s41598-018-27517-8

	Conic shapes have higher sensitivity than cylindrical ones in nanopore DNA sequencing
	Tu, Bin 1; Bai, Shiyang 2; Lu, Benzhuo2,4 ; Fang, Qiaojun 1,3,4,5
	2018-06-14
发表期刊	SCIENTIFIC REPORTS
ISSN	2045-2322
卷号	8 页码:11
摘要	Nanopores have emerged as helpful research tools for single molecule detection. Through continuum modeling, we investigated the effects of membrane thickness, nanopore size, and pore shape on current signal characteristics of DNA. The simulation results showed that, when reducing the pore diameter, the amplitudes of current signals of DNA increase. Moreover, we found that, compared to cylindrically shaped nanopores, conical-shaped nanopores produce greater signal amplitudes from biomolecules translocation. Finally, we demonstrated that continuum model simulations for the discrimination of DNA and RNA yield current characteristics approximately consistent with experimental measurements and that A-T and G-C base pairs can be distinguished using thin conical solid-state nanopores. Our study not only suggests that computational approaches in this work can be used to guide the designs of nanopore for single molecule detection, but it also provides several possible ways to improve the current amplitudes of nanopores for better resolution.
DOI	10.1038/s41598-018-27517-8
语种	英语
资助项目	National Key Research and Development Program of China[2016YFB0201304] ; China NSF[NSFC 21573274] ; China NSF[11771435] ; "Strategic Priority Research Program" of the Chinese Academy of Sciences[XDA09040300] ; 100 Talents Program of the Chinese Academy of Sciences[Y4362911ZX] ; National Natural Science Foundation of China[NSFC 11601101]
WOS研究方向	Science & Technology - Other Topics
WOS类目	Multidisciplinary Sciences
WOS记录号	WOS:000435338100012
出版者	NATURE PUBLISHING GROUP
引用统计
文献类型	期刊论文
条目标识符	http://ir.amss.ac.cn/handle/2S8OKBNM/30489
专题	计算数学与科学工程计算研究所
通讯作者	Lu, Benzhuo; Fang, Qiaojun
作者单位	1.Natl Ctr Nanosci & Technol, CAS Key Lab Biomed Effects Nanomat & Nanosafety, CAS Ctr Excellence Nanosci, Beijing 100190, Peoples R China 2.Chinese Acad Sci, Acad Math & Syst Sci, Beijing 100190, Peoples R China 3.Beijing Key Lab Ambient Particles Hlth Effects &, Beijing 100190, Peoples R China 4.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 5.Sino Danish Ctr Educ & Res, Beijing 101408, Peoples R China
推荐引用方式 GB/T 7714	Tu, Bin,Bai, Shiyang,Lu, Benzhuo,et al. Conic shapes have higher sensitivity than cylindrical ones in nanopore DNA sequencing[J]. SCIENTIFIC REPORTS,2018,8:11.
APA	Tu, Bin,Bai, Shiyang,Lu, Benzhuo,&Fang, Qiaojun.(2018).Conic shapes have higher sensitivity than cylindrical ones in nanopore DNA sequencing.SCIENTIFIC REPORTS,8,11.
MLA	Tu, Bin,et al."Conic shapes have higher sensitivity than cylindrical ones in nanopore DNA sequencing".SCIENTIFIC REPORTS 8(2018):11.