A novel approach to measure all rate constants in the simplest enzyme kinetics model

doi:10.1007/s10910-008-9460-x

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	A novel approach to measure all rate constants in the simplest enzyme kinetics model
	Li, Banghe1,2 ; Li, Bo1,2,3 ; Shen, Yuefeng 1,2,3
	2009-06-01
发表期刊	JOURNAL OF MATHEMATICAL CHEMISTRY
ISSN	0259-9791
卷号	46 期号:1 页码:290-301
摘要	Enzymes play vital roles in life processes. Almost all biochemical reactions are mediated by enzymes. The rate constants of enzyme kinetics are the most important parameters for the reactions catalyzed by enzymes. In 1902, Adrian Brown proposed a simple single-substrate-single-product model which contains only three rate constants k (1), k (-1) and k (2). So far, biologists can measure the Michaelis constant K (M) and the catalytic constant k (cat) , which actually is equal to k (2), according to Michaelis-Menten equation. Using temperature jump method or transient state kinetics, k (1), k (-1) and k (2) can be determined. However, these methods are complicated. In this article, we design a novel simple method that could determine the rate constants k (1) and k (-1) based on knowing k (cat) and K (M) . Our numerical experiments show that the three rate constants can be calculated rather precisely. Hence, we believe that biochemists could design experiments to measure the rate constants based on our method.
关键词	Michaelis constant Catalytic constant Michaelis-Menten equation
DOI	10.1007/s10910-008-9460-x
语种	英语
资助项目	National Natural Science Foundation of China (NSFC)[10771206] ; 973 project[2004CB318000]
WOS研究方向	Chemistry ; Mathematics
WOS类目	Chemistry, Multidisciplinary ; Mathematics, Interdisciplinary Applications
WOS记录号	WOS:000264886100018
出版者	SPRINGER
引用统计
文献类型	期刊论文
条目标识符	http://ir.amss.ac.cn/handle/2S8OKBNM/7422
专题	系统科学研究所
通讯作者	Li, Banghe
作者单位	1.Chinese Acad Sci, Ctr Bioinformat, Acad Math & Syst Sci, Beijing 100190, Peoples R China 2.Chinese Acad Sci, Key Lab Math Mechanizat, Acad Math & Syst Sci, Beijing 100190, Peoples R China 3.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Li, Banghe,Li, Bo,Shen, Yuefeng. A novel approach to measure all rate constants in the simplest enzyme kinetics model[J]. JOURNAL OF MATHEMATICAL CHEMISTRY,2009,46(1):290-301.
APA	Li, Banghe,Li, Bo,&Shen, Yuefeng.(2009).A novel approach to measure all rate constants in the simplest enzyme kinetics model.JOURNAL OF MATHEMATICAL CHEMISTRY,46(1),290-301.
MLA	Li, Banghe,et al."A novel approach to measure all rate constants in the simplest enzyme kinetics model".JOURNAL OF MATHEMATICAL CHEMISTRY 46.1(2009):290-301.