Background: Module detection is widely used to analyze and visualize biological networks. A number of methods and tools have been developed to achieve it. Meanwhile, bipartite module detection is also very useful for mining and analyzing bipartite biological networks and a few methods have been developed for it. However, there is few userfriendly toolkit for this task. Methods: To this end, we develop an online web toolkit BMTK, which implements seven existing methods. Results: BMTK provides a uniform operation platform and visualization function, standardizes input and output format, and improves algorithmic structure to enhance computing speed.We also apply this toolkit onto a drug-target bipartite network to demonstrate its effectiveness. Conclusions: BMTK will be a powerful tool for detecting bipartite modules in diverse bipartite biological networks. Availability: The web application is freely accessible at http://www.zhanglabtools.net/BMTK.