Src activation decouples cell division orientation from cell geometry in mammalian cells

doi:10.1016/j.biomaterials.2018.03.052

CSpace > 系统科学研究所

	Src activation decouples cell division orientation from cell geometry in mammalian cells
	Sun, Xiaoyan 1,2,3,4; Qi, Hongsheng5 ; Zhang, Xiuzhen 1,2,3; Li, Li 1,2,3,6; Zhang, Jiaping 1,2,3; Zeng, Qunli 1,2,3; Laszlo, George S.7; Wei, Bo 8; Li, Tianhong 9; Jiang, Jianxin 10; Mogilner, Alex 11; Fu, Xiaobing 4; Zhao, Min 1,2,3
	2018-07-01
发表期刊	BIOMATERIALS
ISSN	0142-9612
卷号	170 页码:82-94
摘要	Orientation of cell division plane plays a crucial role in morphogenesis and regeneration. Misoriented cell division underlies many important diseases, such as cancer. Studies with Drosophila and C. elegance models show that Src, a proto-oncogene tyrosine-protein kinase, is a critical regulator of this aspect of mitosis. However, the role for Src in controlling cell division orientation in mammalian cells is not well understood. Using genetic and pharmacological approaches and two extracellular signals to orient cell division, we demonstrated a critical role for Src. Either knockout or pharmacological inhibition of Src would retain the fidelity of cell division orientation with the long-axis orientation of mother cells. Conversely, re-expression of Src would decouple cell division orientation from the pre-division orientation of the long axis of mother cells. Cell division orientation in human breast and gastric cancer tissues showed that the Src activation level correlated with the degree of mitotic spindle misorientation relative to the apical surface. Examination of proteins associated with cortical actin revealed that Src activation regulated the accumulation and local density of adhesion proteins on the sites of cell-matrix attachment. By analyzing division patterns in the cells with or without Src activation and through use of a mathematical model, we further support our findings and provide evidence for a previously unknown role for Src in regulating cell division orientation in relation to the pre-division geometry of mother cells, which may contribute to the misoriented cell division. (C) 2018 Elsevier Ltd. All rights reserved.
关键词	Cell division orientation Src Cell geometry Mitosis Cell polarity
DOI	10.1016/j.biomaterials.2018.03.052
语种	英语
资助项目	NIH[1R01EY019101] ; AFOSR[FA9550-16-1-0052] ; Research to Prevent Blindness ; NSFC[30901564] ; NSFC[81101883] ; NSFC[81721092] ; NSFC[81372067] ; NSFC[81230041] ; NSFC[81121004] ; NSFC[81421064] ; National Basic Science and Development Programme (973 Programme)[2012CB518105] ; Beijing Novel Program[2008B53] ; Beijing Novel Program[2009A38] ; National Key R&D Program of China[2017YFC1103304] ; National Key R&D Program of China[2016YFA0101000] ; National Key R&D Program of China[2016YFA0101002]
WOS研究方向	Engineering ; Materials Science
WOS类目	Engineering, Biomedical ; Materials Science, Biomaterials
WOS记录号	WOS:000432903300008
出版者	ELSEVIER SCI LTD
引用统计
文献类型	期刊论文
条目标识符	http://ir.amss.ac.cn/handle/2S8OKBNM/30451
专题	系统科学研究所
通讯作者	Fu, Xiaobing; Zhao, Min
作者单位	1.Univ Calif Davis, Inst Regenerat Cures, Davis, CA 95616 USA 2.Univ Calif Davis, Dept Dermatol, Davis, CA 95616 USA 3.Univ Calif Davis, Dept Ophthalmol, Davis, CA 95616 USA 4.Chinese Peoples Liberat Army Gen Hosp, Postgrad Med Sch, Inst Basic Med Sci, Trauma Ctr,Wound Healing & Cell Biol Lab, 28 Fu Xing Rd, Beijing 100853, Peoples R China 5.Chinese Acad Sci, Acad Math & Syst Sci, Key Lab Syst & Control, 55 Zhongguancun East Rd, Beijing 100190, Peoples R China 6.Third Mil Med Univ, Doping Hosp, Dept Resp Dis, Chongqing 400042, Peoples R China 7.Fred Hutchinson Canc Res Ctr, Clin Res Div, 1100 Fairview Ave, Seattle, WA USA 8.Chinese Peoples Liberat Army Gen Hosp, Dept Gen Surg, 28 Fu Xing Rd, Beijing 100853, Peoples R China 9.Univ Calif Davis, Comprehens Canc Ctr, Div Hematol Oncol, 4501 X St 3016, Sacramento, CA 95817 USA 10.Third Mil Med Univ, Inst Surg, Doping Hosp, State Key Lab Trauma Burns & Combined Injury Res, Chongqing 400042, Peoples R China 11.NYU, Dept Biol, Courant Inst, 251 Mercer St, New York, NY 10003 USA
推荐引用方式 GB/T 7714	Sun, Xiaoyan,Qi, Hongsheng,Zhang, Xiuzhen,et al. Src activation decouples cell division orientation from cell geometry in mammalian cells[J]. BIOMATERIALS,2018,170:82-94.
APA	Sun, Xiaoyan.,Qi, Hongsheng.,Zhang, Xiuzhen.,Li, Li.,Zhang, Jiaping.,...&Zhao, Min.(2018).Src activation decouples cell division orientation from cell geometry in mammalian cells.BIOMATERIALS,170,82-94.
MLA	Sun, Xiaoyan,et al."Src activation decouples cell division orientation from cell geometry in mammalian cells".BIOMATERIALS 170(2018):82-94.