Raman Spectroscopy in Colorectal Cancer Diagnostics: Comparison of PCA-LDA and PLS-DA Models

doi:10.1155/2016/1603609

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	Raman Spectroscopy in Colorectal Cancer Diagnostics: Comparison of PCA-LDA and PLS-DA Models
	Liu, Wenjing 1,2; Sun, Zhaotian 3; Chen, Jinyu 4; Jing, Chuanbo 3
	2016
发表期刊	JOURNAL OF SPECTROSCOPY
ISSN	2314-4920
页码	6
摘要	Raman spectra of human colorectal tissue samples were employed to diagnose colorectal cancer. High-quality Raman spectra were acquired from normal and cancerous colorectal tissues from 81 patients. Subtle Raman variations, such as for peaks at 1134 cm(-1) (protein, C-C/C-N stretching) and 1297 cm(-1) (lipid, C-H-2 twisting), were observed between normal and cancerous colorectal tissues. The average peak intensity at 1134 and 1297 cm(-1) was increased from approximately 235 and 72 in the normal group, respectively, to 315 and 273 in the cancer group. The variations of Raman spectra reflected the changes of cell molecules during canceration. The multivariate statistical methods of principal component analysis-linear discriminant analysis (PCA-LDA) and partial least-squares-discriminant analysis (PLS-DA), together with leave-one-patient-out cross-validation, were employed to build the discriminationmodel. PCA-LDA was used to evaluate the capability of this approach for classifying colorectal cancer, resulting in a diagnostic accuracy of 79.2%. Further PLS-DA modeling yielded a diagnostic accuracy of 84.3% for colorectal cancer detection. Thus, the PLS-DA model is preferable between the two to discriminate cancerous from normal tissues. Our results demonstrate that Raman spectroscopy can be used with an optimized multivariate data analysis model as a sensitive diagnostic alternative to identify pathological changes in the colon at the molecular level.
DOI	10.1155/2016/1603609
语种	英语
资助项目	Strategic Priority Research Program of the Chinese Academy of Sciences[XDB14020201]
WOS研究方向	Biochemistry & Molecular Biology ; Spectroscopy
WOS类目	Biochemical Research Methods ; Spectroscopy
WOS记录号	WOS:000390153900001
出版者	HINDAWI PUBLISHING CORP
引用统计
文献类型	期刊论文
条目标识符	http://ir.amss.ac.cn/handle/2S8OKBNM/24395
专题	中国科学院数学与系统科学研究院
通讯作者	Jing, Chuanbo
作者单位	1.Chinese Acad Sci, Res Ctr Ecoenvironm Sci, State Key Lab Environm Chem & Ecotoxicol, Beijing 100085, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.4 Hosp, Jinan 250031, Peoples R China 4.Chinese Acad Sci, Acad Math & Syst Sci, Natl Ctr Math & Interdisciplinary Sci, Beijing 100190, Peoples R China
推荐引用方式 GB/T 7714	Liu, Wenjing,Sun, Zhaotian,Chen, Jinyu,et al. Raman Spectroscopy in Colorectal Cancer Diagnostics: Comparison of PCA-LDA and PLS-DA Models[J]. JOURNAL OF SPECTROSCOPY,2016:6.
APA	Liu, Wenjing,Sun, Zhaotian,Chen, Jinyu,&Jing, Chuanbo.(2016).Raman Spectroscopy in Colorectal Cancer Diagnostics: Comparison of PCA-LDA and PLS-DA Models.JOURNAL OF SPECTROSCOPY,6.
MLA	Liu, Wenjing,et al."Raman Spectroscopy in Colorectal Cancer Diagnostics: Comparison of PCA-LDA and PLS-DA Models".JOURNAL OF SPECTROSCOPY (2016):6.