N-Linked Glycopeptide Identification Based on Open Mass Spectral Library Search

doi:10.1155/2018/1564136

CSpace > 应用数学研究所

	N-Linked Glycopeptide Identification Based on Open Mass Spectral Library Search
	An, Zhiwu 1,2; Shu, Qingbo 2,3; Lv, Hao 1,2,4; Shu, Lian 2,3; Wang, Jifeng 3; Yang, Fuquan 2,3; Fu, Yan1,2
	2018
发表期刊	BIOMED RESEARCH INTERNATIONAL
ISSN	2314-6133
页码	11
摘要	Confident characterization of intact glycopeptides is a challenging task in mass spectrometry-based glycoproteomics due to microheterogeneity of glycosylation, complexity of glycans, and insufficient fragmentation of peptide bones. Open mass spectral library search is a promising computational approach to peptide identification, but its potential in the identification of glycopeptides has not been fully explored. Here we present pMatchGlyco, a new spectral library search tool for intact N-linked glycopeptide identification using high-energy collisional dissociation (HCD) tandem mass spectrometry (MS/MS) data. In pMatchGlyco, (1) MS/MS spectra of deglycopeptides are used to create spectral library, (2) MS/MS spectra of glycopeptides are matched to the spectra in library in an open (precursor tolerant) manner and the glycans are inferred, and (3) a false discovery rate is estimated for top-scored matches above a threshold. The efficiency and reliability of pMatchGlyco were demonstrated on a data set of mixture sample of six standard glycoproteins and a complex glycoprotein data set generated from human cancer cell line OVCAR3.
DOI	10.1155/2018/1564136
语种	英语
资助项目	International S&T Cooperation Program of China[2014DFB30010] ; National Key R & D Program of China[SQ2018YFA050014] ; Strategic Priority Research Programs of the Chinese Academy of Sciences[XDB13040600] ; Strategic Priority Research Programs of the Chinese Academy of Sciences[XDA12030202] ; National Key Basic Research Program of China[2015CB554406] ; National Natural Science Foundation of China[31670185] ; National Natural Science Foundation of China[91640112] ; NCMIS CAS
WOS研究方向	Biotechnology & Applied Microbiology ; Research & Experimental Medicine
WOS类目	Biotechnology & Applied Microbiology ; Medicine, Research & Experimental
WOS记录号	WOS:000442867000001
出版者	HINDAWI LTD
引用统计
文献类型	期刊论文
条目标识符	http://ir.amss.ac.cn/handle/2S8OKBNM/31102
专题	应用数学研究所
通讯作者	Yang, Fuquan; Fu, Yan
作者单位	1.Chinese Acad Sci, Acad Math & Syst Sci, Natl Ctr Math & Interdisciplinary Sci, Key Lab Random Complex Struct & Data Sci, Beijing 100101, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Chinese Acad Sci, Inst Biophys, Lab Prote, Beijing 100101, Peoples R China 4.Chinese Acad Sci, Comp Network Informat Ctr, Beijing 100101, Peoples R China
推荐引用方式 GB/T 7714	An, Zhiwu,Shu, Qingbo,Lv, Hao,et al. N-Linked Glycopeptide Identification Based on Open Mass Spectral Library Search[J]. BIOMED RESEARCH INTERNATIONAL,2018:11.
APA	An, Zhiwu.,Shu, Qingbo.,Lv, Hao.,Shu, Lian.,Wang, Jifeng.,...&Fu, Yan.(2018).N-Linked Glycopeptide Identification Based on Open Mass Spectral Library Search.BIOMED RESEARCH INTERNATIONAL,11.
MLA	An, Zhiwu,et al."N-Linked Glycopeptide Identification Based on Open Mass Spectral Library Search".BIOMED RESEARCH INTERNATIONAL (2018):11.