A regulatory insertion-deletion polymorphism in the FADS gene cluster influences PUFA and lipid profiles among Chinese adults: a population-based study

doi:10.1093/ajcn/nqy063

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	A regulatory insertion-deletion polymorphism in the FADS gene cluster influences PUFA and lipid profiles among Chinese adults: a population-based study
	Li, Peiqin 1; Zhao, Jing 1; Kothapalli, Kumar S. D.2,3; Li, Xiang 1; Li, Hui 1; Han, Yuxuan 1; Mi, Shengquan 4; Zhao, Wenhua 5; Li, Qizhai6 ; Zhang, Hong 7; Song, Yiqing 8; Brenna, J. Thomas 2,3; Gao, Ying 1
	2018-06-01
发表期刊	AMERICAN JOURNAL OF CLINICAL NUTRITION
ISSN	0002-9165
卷号	107 期号:6 页码:867-875
摘要	Background: Arachidomc acid (AA) is the major polyunsaturated fatty acid (PUFA) substrate for potent eicosanoid signaling to modulate inflammation and thrombosis and is controlled in part by tissue abundance. Fatty acid desaturase 1 (FADS1) catalyzes synthesis of omega-6 (n-6) AA and n-3 eicosapentaenoic acid (EPA). The rs66698963 polymorphism, a 22-base pair (bp) insertion-deletion 137 bp downstream of a sterol regulatory element in FADS2 intron 1, mediates expression of FADS1 in vitro, as well as exerting positive selection in several human populations. The associations between the polymorphism rs66698963 and plasma PUFAs as well as disease phenotypes are unclear. Objective: This study aimed to evaluate the relation between rs66698963 genotypes and plasma PUFA concentrations and blood lipid profiles. Design: Plasma fatty acids were measured from a single sample obtained at baseline in 1504 healthy Chinese adults aged between 35 and 59 y with the use of gas chromatography. Blood lipids were measured at baseline and a second time at the 18-mo follow-up. The rs66698963 genotype was determined by using agarose gel electrophoresis. Linear regression and logistic regression analyses were performed to assess the association between genotype and plasma PUFAs and blood lipids. Results: A shift from the precursors linoleic acid and a-linolemc acid to produce AA and EPA, respectively, was observed, consistent with FADS 1 activity increasing in the order of genotypes D/D to I/D to I/I. For FI compared with D/D carriers, plasma concentrations of n-6 AA and the ratio of AA to n-3 EPA plus docosahexaenoic acid (DHA) were 57% and 32% higher, respectively. Carriers of the deletion (D) allele of rs66698963 tended to have higher triglycerides (beta= 0.008; SE: 0.009; P = 0.05) and lower HDL cholesterol (beta = -0.008; SE: 0.004; P = 0.02) than carriers of the insertion (I) allele. Conclusions: The rs66698963 genotype is significantly associated with AA concentrations and AA to EPA+DHA ratio, reflecting basal risk of inflammatory and related chronic disease phenotypes, and is correlated with the risk of dyslipidemia. This trial was registered at chictr.org.cn as ChiCTR-EOC-17012759. Am J Clin Nutr 2018;107:867-875.
关键词	dyslipidemia gene polymorphism fatty acid desaturases insertion-deletion long-chain polyunsaturated fatty acids
DOI	10.1093/ajcn/nqy063
语种	英语
资助项目	National Key Research and Development Plan of Chinese Academy of Sciences[2016YFD0400200] ; 100 Talented Plan of Chinese Academy of Sciences
WOS研究方向	Nutrition & Dietetics
WOS类目	Nutrition & Dietetics
WOS记录号	WOS:000435459400005
出版者	OXFORD UNIV PRESS
引用统计
文献类型	期刊论文
条目标识符	http://ir.amss.ac.cn/handle/2S8OKBNM/30548
专题	系统科学研究所
通讯作者	Gao, Ying
作者单位	1.Univ Chinese Acad Sci, Chinese Acad Sci, Shanghai Inst Biol Sci, Key Lab Nutr & Metab,Inst Nutr Sci, Shanghai, Peoples R China 2.Cornell Univ, Div Nutr Sci, Ithaca, NY 14853 USA 3.Univ Texas Austin, Dell Med Sch, Dell Pediat Res Inst, Austin, TX 78712 USA 4.Beijing Union Univ, Coll Biochem Engn, Beijing, Peoples R China 5.Chinese Ctr Dis Control & Prevent, Natl Inst Nutr & Hlth, Beijing, Peoples R China 6.Chinese Acad Sci, Acad Math & Syst Sci, Beijing, Peoples R China 7.Fudan Univ, Sch Life Sci, Inst Biostat, Shanghai, Peoples R China 8.Indiana Univ, Richard M Fairbanks Sch Publ Hlth, Dept Epidemiol, Indianapolis, IN 46204 USA
推荐引用方式 GB/T 7714	Li, Peiqin,Zhao, Jing,Kothapalli, Kumar S. D.,et al. A regulatory insertion-deletion polymorphism in the FADS gene cluster influences PUFA and lipid profiles among Chinese adults: a population-based study[J]. AMERICAN JOURNAL OF CLINICAL NUTRITION,2018,107(6):867-875.
APA	Li, Peiqin.,Zhao, Jing.,Kothapalli, Kumar S. D..,Li, Xiang.,Li, Hui.,...&Gao, Ying.(2018).A regulatory insertion-deletion polymorphism in the FADS gene cluster influences PUFA and lipid profiles among Chinese adults: a population-based study.AMERICAN JOURNAL OF CLINICAL NUTRITION,107(6),867-875.
MLA	Li, Peiqin,et al."A regulatory insertion-deletion polymorphism in the FADS gene cluster influences PUFA and lipid profiles among Chinese adults: a population-based study".AMERICAN JOURNAL OF CLINICAL NUTRITION 107.6(2018):867-875.