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Drosophila TRF2 and TAF9 regulate lipid droplet size and phospholipid fatty acid composition
Fan, Wei1,2; Lam, Sin Man1; Xin, Jingxue2,3; Yang, Xiao1,2; Liu, Zhonghua1; Liu, Yuan1,2; Wang, Yong3; Shui, Guanghou1; Huang, Xun1,2
2017-03-01
Source PublicationPLOS GENETICS
ISSN1553-7404
Volume13Issue:3Pages:21
AbstractThe general transcription factor TBP (TATA-box binding protein) and its associated factors (TAFs) together form the TFIID complex, which directs transcription initiation. Through RNAi and mutant analysis, we identified a specific TBP family protein, TRF2, and a set of TAFs that regulate lipid droplet (LD) size in the Drosophila larval fat body. Among the three Drosophila TBP genes, trf2, tbp and trf1, only loss of function of trf2 results in increased LD size. Moreover, TRF2 and TAF9 regulate fatty acid composition of several classes of phospholipids. Through RNA profiling, we found that TRF2 and TAF9 affects the transcription of a common set of genes, including peroxisomal fatty acid beta-oxidation-related genes that affect phospholipid fatty acid composition. We also found that knockdown of several TRF2 and TAF9 target genes results in large LDs, a phenotype which is similar to that of trf2 mutants. Together, these findings provide new insights into the specific role of the general transcription machinery in lipid homeostasis.
DOI10.1371/journal.pgen.1006664
Language英语
Funding ProjectStrategic Priority Research Program oft he Chinese Academy of Sciences[XDB13030300/0400] ; National Basic Research Program of China[2013CB530606] ; National Basic Research Program of China[2016YFA0500100] ; National Natural Science Foundation of China[11422108] ; National Natural Science Foundation of China[31325018] ; National Natural Science Foundation of China[31571377] ; National Natural Science Foundation of China[31630019]
WOS Research AreaGenetics & Heredity
WOS SubjectGenetics & Heredity
WOS IDWOS:000398043000040
PublisherPUBLIC LIBRARY SCIENCE
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Document Type期刊论文
Identifierhttp://ir.amss.ac.cn/handle/2S8OKBNM/25148
Collection应用数学研究所
Corresponding AuthorHuang, Xun
Affiliation1.Chinese Acad Sci, Inst Genet & Dev Biol, State Key Lab Mol Dev Biol, Beijing, Peoples R China
2.Univ Chinese Acad Sci, Beijing, Peoples R China
3.Chinese Acad Sci, Acad Math & Syst Sci, Natl Ctr Math & Interdisciplinary Sci, Beijing, Peoples R China
Recommended Citation
GB/T 7714
Fan, Wei,Lam, Sin Man,Xin, Jingxue,et al. Drosophila TRF2 and TAF9 regulate lipid droplet size and phospholipid fatty acid composition[J]. PLOS GENETICS,2017,13(3):21.
APA Fan, Wei.,Lam, Sin Man.,Xin, Jingxue.,Yang, Xiao.,Liu, Zhonghua.,...&Huang, Xun.(2017).Drosophila TRF2 and TAF9 regulate lipid droplet size and phospholipid fatty acid composition.PLOS GENETICS,13(3),21.
MLA Fan, Wei,et al."Drosophila TRF2 and TAF9 regulate lipid droplet size and phospholipid fatty acid composition".PLOS GENETICS 13.3(2017):21.
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