KMS Of Academy of mathematics and systems sciences, CAS
A clustering approach to multireference alignment of single-particle projections in electron microscopy | |
Sorzano, C. O. S.1; Bilbao-Castro, J. R.2; Shkolnisky, Y.3; Alcorlo, M.1; Melero, R.1; Caffarena-Fernandez, G.4; Li, M.5; Xu, G.5; Marabini, R.6; Carazo, J. M.1 | |
2010-08-01 | |
发表期刊 | JOURNAL OF STRUCTURAL BIOLOGY |
ISSN | 1047-8477 |
卷号 | 171期号:2页码:197-206 |
摘要 | Two-dimensional analysis of projections of single-particles acquired by an electron microscope is a useful tool to help identifying the different kinds of projections present in a dataset and their different projection directions. Such analysis is also useful to distinguish between different kinds of particles or different particle conformations. In this paper we introduce a new algorithm for performing two-dimensional multireference alignment and classification that is based on a Hierarchical clustering approach using correntropy (instead of the more traditional correlation) and a modified criterion for the definition of the clusters specially suited for cases in which the Signal-to-Noise Ratio of the differences between classes is low. We show that our algorithm offers an improved sensitivity over current methods in use for distinguishing between different projection orientations and different particle conformations. This algorithm is publicly available through the software package Xmipp. (C) 2010 Elsevier Inc. All rights reserved. |
关键词 | Single-particle analysis 2D analysis Multireference analysis Electron microscopy |
DOI | 10.1016/j.jsb.2010.03.011 |
语种 | 英语 |
资助项目 | European Union[FP6-502828] ; European Union[UE-512092] ; 3DEM European network[LSHG-CT-2004-502828] ; ANR[PCV06-142771] ; Spanish Ministerio de Educacion[CSD2006-0023] ; Spanish Ministerio de Educacion[BIO2007-67150-001] ; Spanish Ministerio de Educacion[BIO2007-67150-C03] ; Spanish Ministerio de Ciencia e Innovacion[ACI2009-1022] ; Spanish Fondo de Investigacion Sanitaria[04/0683] ; Comunidad de Madrid[S-GEN-0166-2006] ; European Social Fund ; Ministerio de Ciencia e Innovacion ; NSFC[60773165] ; NSFC[10990013] ; National Heart, Lung, And Blood Institute[R01HL070472] ; [MCI-TIN2008-01117] ; [JA-P06-TIC01426] |
WOS研究方向 | Biochemistry & Molecular Biology ; Biophysics ; Cell Biology |
WOS类目 | Biochemistry & Molecular Biology ; Biophysics ; Cell Biology |
WOS记录号 | WOS:000279151900010 |
出版者 | ACADEMIC PRESS INC ELSEVIER SCIENCE |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://ir.amss.ac.cn/handle/2S8OKBNM/11197 |
专题 | 中国科学院数学与系统科学研究院 |
通讯作者 | Sorzano, C. O. S. |
作者单位 | 1.CSIC, Biocomp Unit, Natl Biotechnol Ctr, Madrid 28049, Spain 2.Univ Almeria, Comp Architecture & Elect Dept, Almeria, Spain 3.Tel Aviv Univ, Dept Appl Math, IL-69978 Tel Aviv, Israel 4.Univ San Pablo CEU, Dept Ingn Sistemas Elect & Telecomunicac, Madrid, Spain 5.Chinese Acad Sci, Inst Computat Math & Sci Engn Comp, Beijing, Peoples R China 6.Univ Autonoma Madrid, Dept Comp Sci, Madrid, Spain |
推荐引用方式 GB/T 7714 | Sorzano, C. O. S.,Bilbao-Castro, J. R.,Shkolnisky, Y.,et al. A clustering approach to multireference alignment of single-particle projections in electron microscopy[J]. JOURNAL OF STRUCTURAL BIOLOGY,2010,171(2):197-206. |
APA | Sorzano, C. O. S..,Bilbao-Castro, J. R..,Shkolnisky, Y..,Alcorlo, M..,Melero, R..,...&Carazo, J. M..(2010).A clustering approach to multireference alignment of single-particle projections in electron microscopy.JOURNAL OF STRUCTURAL BIOLOGY,171(2),197-206. |
MLA | Sorzano, C. O. S.,et al."A clustering approach to multireference alignment of single-particle projections in electron microscopy".JOURNAL OF STRUCTURAL BIOLOGY 171.2(2010):197-206. |
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