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CAN WE USE PBS AS AN ALTERNATIVE TO DEXTRAN IN EKTACYTOMETRIC MEASUREMENT
WEN, ZY; TAO, ZL; CAO, S; ZHAO, YH; HAN, ZS; LI, YM; LU, SH; WANG, HR; XU, JL; LI, G; YU, GF; WU, BJ
1991
Source PublicationCLINICAL HEMORHEOLOGY
ISSN0271-5198
Volume11Issue:5Pages:459-464
AbstractIn order to find a new suspending medium for Ektacytometric measurement, comparisons of measured rheological properties of erythrocyte suspension in different buffers were carried out. Two kinds of buffer were used, e. g. PBS and PVP. And four types of erythrocyte were measured by using both suspeneding media, namely: normal rabbit's erythrocytes (RBC). rabbit's RBC hardened by formalin with different concentrations, rabbit's RBC treated by trypsin and human erythrocyte sampled from patients. Microscopic and scanning electron microscopic photographs of erythrocytes rigidizied al constant shear rate were observed. Results showed that (1) for every one of the these four kinds of erythrocytes, measured deformation index (D1) - shear rate (gamma) curves in PBS are similar qualitatively to those measured in PVP. (2) Microscopic and scanning electron microscopic photographs showed that erythrocytes indeed deformed when the shear rate of flow field for PBS was as low as gamma = 20s-1. It indicated that PBS could be used as suspending medium. On the contrary when we used PVP (eta = 2 mPas Fig. 1 [1]) as suspending medium at gamma = 800s-1, no obvious deformation could be obtained by using Ektacytometer. It seems to mean that, as the suspending medium for Ektacytometric measurement, PBS would be better than PVP, especially in low shear stress area. This point of view is different from that of previous works [2] which do not consider PBS as suspending medium of Ektacytometer.
KeywordDEFORMABILITY SUSPENDING MEDIUM EKTACYTOMETRIC
Language英语
WOS Research AreaHematology
WOS SubjectHematology
WOS IDWOS:A1991GM64600022
PublisherPERGAMON-ELSEVIER SCIENCE LTD
Citation statistics
Document Type期刊论文
Identifierhttp://ir.amss.ac.cn/handle/2S8OKBNM/28157
Collection中国科学院数学与系统科学研究院
Affiliation1.BEIJING MED UNIV,DEPT BIOPHYS,BEIJING,PEOPLES R CHINA
2.CHINESE ACAD SCI,INST MATH,BEIJING,PEOPLES R CHINA
Recommended Citation
GB/T 7714
WEN, ZY,TAO, ZL,CAO, S,et al. CAN WE USE PBS AS AN ALTERNATIVE TO DEXTRAN IN EKTACYTOMETRIC MEASUREMENT[J]. CLINICAL HEMORHEOLOGY,1991,11(5):459-464.
APA WEN, ZY.,TAO, ZL.,CAO, S.,ZHAO, YH.,HAN, ZS.,...&WU, BJ.(1991).CAN WE USE PBS AS AN ALTERNATIVE TO DEXTRAN IN EKTACYTOMETRIC MEASUREMENT.CLINICAL HEMORHEOLOGY,11(5),459-464.
MLA WEN, ZY,et al."CAN WE USE PBS AS AN ALTERNATIVE TO DEXTRAN IN EKTACYTOMETRIC MEASUREMENT".CLINICAL HEMORHEOLOGY 11.5(1991):459-464.
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